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zic3 antisense RNA probe primers

eagle-i ID

http://montana.eagle-i.net/i/0000012e-96ba-4143-55f8-6fb280000000

Resource Type

  1. Primer

Properties

  1. Resource Description
    T7 promoter-containing PCR products were synthesized from stage 18 chick cDNA. The gel-purified PCR products were used as templates for synthesis of antisense RNA probes using T7 polymerase. Note: -TAATACGACTCACTATA- sequence within reverse primer is the T7 RNA polymerase promoter .
  2. Contact
    Merzdorf, Christa
  3. Entrez Gene ID
    374276
  4. Related Publication or Documentation
    Expression of the zic1, zic2, zic3, and zic4 genes in early chick embryos.
  5. Related Technique
    In situ hybridization
  6. Entrez Gene Symbol
    ZIC3
  7. Sequence
    Forward: 5'-CAGCAAGGACTCCACGAAAAC-3'
  8. Sequence
    Reverse: 5'-CTAATACGACTCACTATAGGCGACCCCATCAGATGAGAAT-3' (ca. 730 bp; little 3' coding region and mostly 3'UTR)
  9. Location
    Merzdorf Lab
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    kcorday
  3. contributor
    nvasilevsky
  4. created
    2011-03-08T12:49:22.755-06:00
  5. creator
    qking (Quinton King)
  6. modified
    2012-03-01T19:36:50.495-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016