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zic1 cDNA clone for antisense RNA probe generation

eagle-i ID

http://montana.eagle-i.net/i/0000012e-96d3-e64e-55f8-6fb280000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    zic1 cDNA
  2. Resource Description
    For zic1, PCR products were synthesized from stage 18 chick cDNA, which was obtained by reverse transcription of isolated total RNA. These PCR products were TA cloned into pGEM-T (Promega) and transcribed from the resulting plasmids to generate antisense RNA probes. zic1 forward: 5'-GCGCTAAAACAAAACAGCGA-3' zic1 reverse: 5'-CTGTATTTACAAGAGGGAGTGGG-3' (497 bp in 3'UTR)
  3. Contact
    Merzdorf, Christa
  4. Construct Backbone
    pGEM-T
  5. Related Publication or Documentation
    Expression of the zic1, zic2, zic3, and zic4 genes in early chick embryos.
  6. Related Technique
    In situ hybridization
  7. Location
    Merzdorf Lab
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    tjohnson
  3. created
    2011-03-08T13:01:45.010-06:00
  4. creator
    qking (Quinton King)
  5. modified
    2016-08-25T13:58:53.573-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016