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zic1 cDNA clone for antisense RNA probe generation

eagle-i ID


Resource Type

  1. Plasmid


  1. Construct Insert
    zic1 cDNA
  2. Resource Description
    For zic1, PCR products were synthesized from stage 18 chick cDNA, which was obtained by reverse transcription of isolated total RNA. These PCR products were TA cloned into pGEM-T (Promega) and transcribed from the resulting plasmids to generate antisense RNA probes. zic1 forward: 5'-GCGCTAAAACAAAACAGCGA-3' zic1 reverse: 5'-CTGTATTTACAAGAGGGAGTGGG-3' (497 bp in 3'UTR)
  3. Contact
    Merzdorf, Christa
  4. Construct Backbone
  5. Related Publication or Documentation
    Expression of the zic1, zic2, zic3, and zic4 genes in early chick embryos.
  6. Related Technique
    In situ hybridization
  7. Location
    Merzdorf Lab
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
  3. created
  4. creator
    qking (Quinton King)
  5. modified
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