eagle-i Montana State UniversityMontana State University
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

CHO-K1 FPR1 wild-type

eagle-i ID

http://montana.eagle-i.net/i/00000131-00dd-5f7f-44b9-3efb80000000

Resource Type

  1. Cell line

Properties

  1. Resource Description
    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.
  2. Contact
    Miettinen, Heini
  3. Related Publication or Documentation
    Agonist-dependent phosphorylation of the formyl peptide receptor is regulated by the membrane proximal region of the cytoplasmic tail.
  4. Related Publication or Documentation
    Formyl peptide receptor-mediated ERK1/2 activation occurs through G(i) and is not dependent on beta-arrestin1/2.
  5. Related Publication or Documentation
    The ligand binding site of the formyl peptide receptor maps in the transmembrane region.
  6. Related Publication or Documentation
    Identification of putative sites of interaction between the human formyl peptide receptor and G protein.
  7. Related Publication or Documentation
    A single amino acid substitution (N297A) in the conserved NPXXY sequence of the human N-formyl peptide receptor results in inhibition of desensitization and endocytosis, and a dose-dependent shift in p42/44 mitogen-activated protein kinase activation and chemotaxis.
  8. Related Publication or Documentation
    Activation and nuclear translocation of ERK1/2 by the formyl peptide receptor is regulated by G protein and is not dependent on beta-arrestin translocation or receptor endocytosis.
  9. Expressed Construct(s)
    FPR1 wild-type
  10. Related Technique
    Immunofluorescence microscopy assay
  11. Related Technique
    Transfection
  12. Location
    Miettinen Lab
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    qking (Quinton King)
  3. created
    2011-07-06T14:14:21.851-05:00
  4. creator
    qking (Quinton King)
  5. modified
    2011-07-06T22:18:41.740-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016