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FACS Core Laboratory

Summary:

Laboratory performs fluorescence cytometry services related to Immunology, Infectious Disease, and Immunostimulatory Compounds.

Affiliations:

People:

Resources:

Instruments

  • BD FACSAria ( Flow cytometer sorter )

    "The BD FACSAria flow cytometer is the first fixed-alignment benchtop high- speed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of 7 fluorescent markers and two scatter parameters at a time."

  • BD FACSCalibur flow cytometer ( Flow cytometer sorter )

    "The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system." Instrument has 2 lasers and can analyze up to 4 colors.

  • BD FACSCalibur flow cytometer with HTS ( Flow cytometer sorter )

    "The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system." Instrument has 2 lasers and can analyze up to 4 colors. Instrument has BD HTS 96-well plate reader add-on.

  • BD FACSCanto flow cytometer ( Flow cytometer sorter )

    Configured with 2 lasers to detect up to 6 colors.

  • BD HTS 96-well plate reader ( Microplate reader )

  • BD LSR II flow cytometer ( Flow cytometer )

    The BD LSR II flow cytometer is configured with 2 fixed-aligned, air-cooled lasers to detect up to 8 colors.

Protocols

Services

  • Assisted support ( Training service )

    Support and training in instruments and techniques provided by the FACS Core Laboratory.

  • Cell sorting ( Material processing service )

    Cell sorting is provided by the facility personnel. Service fees apply. Contact lab for details.

  • Equipment access ( Access service )

    Provides access to facility instrumentation.

Software

  • BD CellQuest Pro ( Software )

    "BD CellQuest Pro software allows you to acquire and analyze data from your flow cytometer on a Macintosh® computer. Working in the BD CellQuest Pro Experiment document window, you can create several types of plots, including multicolor contour plots and overlaid histograms, and you can generate statistics for dot plots, histograms, density plots, 3D plots, and contour plots. The versatile graphics tool palette helps you produce presentation-quality documents. You can also save everything in an Experiment document, including plots, regions, gates, markers, statistics, calculated expressions, Browser contents, annotations, and color preferences, and then restore it all later."

  • BD FACSDiva ( Software )

    "BD FACSDiva™ software is a collection of rich tools for flow cytometer and application setup, data acquisition, and analysis that help streamline flow cytometry workflow for today's busy laboratory. BD FACSDiva software offers built-in features for consistent and maintainable daily cytometer setup that supports reproducible data acquisition and reliable results."

  • FlowJo ( Software )

    Flow cytometry data and statistical analysis software.

  • ModFit LT ( Software )

    ModFit provides open-ended modeling of flow cytometry histograms and can be used for DNA analysis. Has built in models for use with cell-tracking dye studies and synchronized cell lines.


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Last updated: 2011-04-06T00:37:20.167-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016