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Microscopy Core Facility

Summary:

"The Center for Biofilm Engineering Microscopy Facility is a research-only facility on the MSU campus, located on the third floor of the EPS building. The Microscopy Facilities Manager trains and assists faculty, research staff and students with capturing images of samples via optical microscopy and fluorescent confocal microscopy. The microscopy facilities include three separate laboratories - the Optical Microscopy Lab, the Confocal Microscopy Lab, and the Microscope Resource Room and Digital Imaging Lab."

Large inventory of fluorescent stains. Inquire for availability, applications and collaborations.

People:

Resources:

Instruments

  • Leica Cryostat ( Cryostat )

    A cryostat is a microtome for sectioning frozen tissues/samples, typically for histological applications.

  • Microbeam System ( Laser capture microdissection microscope )

    Laser capture microdissection microscopy utilizes a UV laser pulsed microbeam to excise a cell or cells of interest from slide-mounted tissue. The isolated tissue is then recovered by laser pressure catapult into a microfuge tube cap. The UV wavelength of the laser is too long to damage polynucleotides and proteins. LCM is suitable for most routinely prepared cell and tissues samples, including single cells and microbial applications.

  • Nikon Eclipse E-800 Microscope ("Lefty") ( Optical microscope )

    Nomarski Differential Interference Contrast; FITC; TRITC; DAPI; CTC; ELF-97; CY5; cfp; B2A cube; Hamamatsu CCD Video Camera add-on.

  • Nikon Eclipse E-800 Microscope ("Righty") ( Optical microscope )

    Nomarski Differential Interference Contrast; FITC; TRITC; DAPI; CTC; ELF-97; CY5; cfp; B2A cube.

  • Nikon Stereo Microscope ( Stereo microscope )

    Infinity 2 Color Camera add-on.

  • Photometrics CoolSnapEZ CCD Camera ( Charge-coupled device camera )

  • Photometrics CoolSnapfx CCD Camera ( Charge-coupled device camera )

  • Spectra-Physics MaiTai 2-Photon Infra-Red laser/detector ( Two-photon laser/detector )

  • TCS-NT Microscope ( Laser scanning confocal microscope )

    2 Laser; 488 nm excitation; 568 nm excitation.

  • TCS-SP2 AOBS Microscope ( Laser scanning confocal microscope )

    7 Laser; 458 nm excitation;476 nm excitation; 488 nm excitation; 496 nm excitation; 514 nm excitation; 543 nm excitation; 633 nm excitation; Spectra-Physics MaiTai 2-photon infra-red laser and detector add-on.

Protocols

Services

  • Microscopy facility training and access ( Access service )

    "The CBE Microscopy Facility is a research-only facility. That is, the facilities manager trains all users to do their own imaging, and is not available on an imaging-for-hire basis. If you think to would like to use the facility to do your research, first get an idea of what approach you might take, from other researchers or published literature, for example, find out which fluorescence dyes, probes or proteins you might use. Then contact the facilities manager to discuss the work you would like to do. We will find out then if we have the right equipment for you, and if we are a good match. If so, you will schedule training sessions on the epifluorescent microscopes and software, which are generally two 2-hour sessions, one-on-one only. If you have adequate experience on epifluorescent microscopes and software, this will be obvious, and we will adjust the sessions accordingly. If you need to use the confocal microscope, the same training format will apply, and follows the epifluorescent scope training. All users begin facilities use on the epifluorescent microscopes."

    Significant expertise is available with respect to biofilm imaging. $1500/person/year.

Software

  • Imaris ( Software )

    "Imaris is Bitplane’s core scientific software module that delivers all the necessary functionality for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size. Conveniently load, process and visualize data and images acquired from almost any confocal and wide field microscope to gain new and groundbreaking insight from your image data."

  • MetaMorph ( Software )

    "MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years."

  • MetaVue ( Software )

    "The MetaVue™ Research Imaging Software is the cost-effective solution for basic imaging applications such as digital photography, multi-wavelength fluorescence, image processing, and analysis. MetaVue Software is a simple, easy-to-use system for acquiring and processing images, performing graphics functions, and archiving and retrieving images."


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Last updated: 2011-04-27T16:04:56.854-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016