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Proteomics Core Laboratory

Summary:

The mission of the Mass Spectrometry and Proteomics Facility is to seed mass spectrometry and proteomics technology to research labs at Montana State University and affiliated programs.

People:

Resources:

Instruments

Protocols

Services

  • 2-D gel (access) ( Access service )

    Provides access to equipment necessary for 2D protein electrophoresis.

  • 2-D gel (facility run) ( Material analysis service )

    2D protein electrophoresis performed by facility staff. Inquire for pricing.

  • GCMS (access) ( Access service )

    Provides access to Gas Chromatography-Mass Spectrometry instrumentation.

  • GCMS (facility run) ( Material analysis service )

    Gas Chromatography-Mass Spectrometry performed by facility staff. Inquire for pricing.

  • HPLC-micrOTOF (facility run) ( Material analysis service )

    High Performance Liquid Chromatography-micrOTOF; samples run by facility staff.

  • MALDI (access) ( Access service )

    Provides access to Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry instrumentation.

  • MALDI (facility run) ( Material analysis service )

    Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry sample analysis performed by facility staff.

  • MicrOTOF (access) ( Access service )

    Provides access to HPLC and MicrOTOF instrumentation.

  • MicrOTOF high resolution (facility run) ( Material analysis service )

    HPLC/MicrOTOF sample analysis performed by facility staff.

  • MicrOTOF low res (facility run) ( Material analysis service )

    HPLC/MicrOTOF sample analysis performed by facility staff.

  • XCT ion trap (access) ( Access service )

    Provides access to LC/MS ion trap instrumentation for sample analysis.

Software

  • Decyder ( Software )

    "DeCyder 2D applies a gel comparison method that introduces zero statistical error, offering reliable data and analysis for 2-D DIGE experiments."

  • Mascot ( Software )

    "Mascot is a powerful search engine which uses mass spectrometry data to identify proteins from primary sequence databases. While a number of similar programs available, Mascot is unique in that it integrates all of the proven methods of searching. These different search methods can be categorised as follows:

    * Peptide Mass Fingerprint in which the only experimental data are peptide mass values.

    * Sequence Query in which peptide mass data are combined with amino acid sequence and composition information. A super-set of a sequence tag query.

    * MS/MS Ion Search using uninterpreted MS/MS data from one or more peptides."

  • PHENYX ( Software )

    "Phenyx enables proteomics scientists to 1) submit MS/MS data and identify peptides and proteins 2) visualize and evaluate results using various dynamic views, 3) manually validate results and compare Phenyx runs and those of other database search engines (like Mascot, SEQUEST and X!Tandem), 4) perform quantitative analysis with the Phenyx quantitation module, and 5) export results and generate reports into various formats and integrate Phenyx into your laboratory workflow.

    Through its import feature, its advanced Results Comparison functionality and its quantitation module, Phenyx goes beyond being a single search engine tool to become a comprehensive identification platform allowing users to import, compare, quantify and validate their Phenyx results but progressively also their Mascot, SEQUEST and X!Tandem results while visualizing them in various levels of granularity and easily exporting their comparative results in one click for publication."

  • Progenesis SameSpots PG240 ( Software )

    "The Progenesis SameSpots analysis workflow allows you to confidently generate 2D analysis results which are accurate and robust."

  • Spectrum Mill ( Software )

    "Large-scale proteome characterization frequently leads to bottlenecks in data interpretation and review. Spectrum Mill for MassHunter Workstation quickly identifies proteins and peptides via fast database searches, with automatic or manual match validation and unique algorithms that minimize false positives. It also offers de novo spectral interpretation for proteins not found in any database. Spectrum Mill software can identify relative abundance differences of twofold or greater without complicated isotope labeling. And Spectrum Mill summarizes and correlates results in ways that provide maximum insight and convenience."

  • X! P3 (Proteolytic Peptide Profiler) ( Software )

  • X!HUNTER ( Software )

  • X!TANDEM ( Software )


Web Links:

Last updated: 2014-10-08T14:04:34.547-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016