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Miettinen Lab

Summary:

Research in our lab is directed toward understanding the function of two neutrophil receptors, the formyl peptide receptor (FPR) and the C5a receptor (C5aR), which play a central role in inflammation. Ligand binding to these receptors leads to important host defense functions such as killing of microorganisms. Unfortunately, neutrophils are also involved in the pathology of various inflammatory conditions. Our goal is to learn more about neutrophil activation by studying the functions of FPR and C5aR.

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Reagents

  • c-Myc-FPR1 ( cDNA plasmid )

    Human FPR1 with c-Myc tag. Fusion constructs with c-Myc were created as indicated in the linked reference.

  • C5aR L318A ( cDNA plasmid )

    Human C5aR with L318A point mutation. Point mutations of human C5aR cDNA were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • C5aR L318A/L319A ( cDNA plasmid )

    Human C5aR with L318A and L319A point mutations. Point mutations of human C5aR cDNA were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • C5aR L319A ( cDNA plasmid )

    Human C5aR with L319A point mutation. Point mutations of human C5aR cDNA were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • C5aR wild-type ( cDNA plasmid )

    Human wild-type C5aR.

  • C5aR-EGFP ( cDNA plasmid )

    Human wild-type C5aR with EGFP tag. Fusion construct with EGFP was created as indicated in the linked reference.

  • CHO-K1 c-Myc-FPR1 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 C5aR L318A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant C5aR. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 C5aR L318A/L319A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant C5aR. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 C5aR L319A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant C5aR. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 C5aR wild-type ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant C5aR. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 C5aR-EGFP ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant C5aR. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 A318S/S319L ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 C124S/C126S ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 C295S ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 C73S ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D106N ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D122-AAA-R123 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D122A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D284N/V285P ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D327P ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D333P ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D71A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D71A-EGFP ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D71N ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 D71N/N297D ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 E321L ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 E321Q ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 F206A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 F291A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 F291Y ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 F33L/A34G ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 F74A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 FPR1 ∆316-322 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 H57S ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 I204Y ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 I204Y/R205A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 I224A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 K143A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L101I/F102H ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L109A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L223A/L240A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L243-AAA-S244 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L243A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 L78A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 N297A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 N297A-EGFP ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 N44A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 P213A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 P298A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 Q258A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R123A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R123G ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R201A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R205A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R241A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 R54A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 S220A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 S287A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 S319L ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 S63C/Y64F ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 S63W ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 T103I ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 T157A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 V105A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 V246A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 W150A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 W254A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 wild-type ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 Y221A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 Y257A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 Y257F ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 Y301A ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 ∆128-135 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 ∆229-234 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 ∆309-315 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 ∆323-329 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1 ∆330-336 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 FPR1-EGFP ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • CHO-K1 HA-FPR1 ( Cell line )

    CHO-K1 cells were transfected with mammalian expression vector pBGSA encoding wild-type and mutant FPR1. Clones were selected in G418 and expression was analyzed by immunofluorescence microscopy.

  • FPR1 A318S/S319L ( cDNA plasmid )

    Human FPR1 with A318S and S319L point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 C124S/C126S ( cDNA plasmid )

    Human FPR1 with C124S and C126S point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 C295S ( cDNA plasmid )

    Human FPR1 with C295S point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 C73S ( cDNA plasmid )

    Human FPR1 with C73S point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D106N ( cDNA plasmid )

    Human FPR1 with D106N point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D122-AAA-R123 ( cDNA plasmid )

    Human FPR1 with D122-AAA-R123 Alanine insertion mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D122A ( cDNA plasmid )

    Human FPR1 with D122A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D284N/V285P ( cDNA plasmid )

    Human FPR1 with D284N and V285P point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D327P ( cDNA plasmid )

    Human FPR1 with D327P point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D333P ( cDNA plasmid )

    Human FPR1 with D333P point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D71A ( cDNA plasmid )

    Human FPR1 with D71A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D71A-EGFP ( cDNA plasmid )

    Human FPR1 with D71A point mutation and an EGFP tag. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing. Fusion constructs with EGFP were created as indicated in the references.

  • FPR1 D71N ( cDNA plasmid )

    Human FPR1 with D71N point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 D71N/N297D ( cDNA plasmid )

    Human FPR1 with D71N/N297D point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 E321L ( cDNA plasmid )

    Human FPR1 with E321L point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 E321Q ( cDNA plasmid )

    Human FPR1 with E321Q point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 F206A ( cDNA plasmid )

    Human FPR1 with F206A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 F291A ( cDNA plasmid )

    Human FPR1 with F291A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 F291Y ( cDNA plasmid )

    Human FPR1 with F291Y point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 F33L/A34G ( cDNA plasmid )

    Human FPR1 with F33L and A34G point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 F74A ( cDNA plasmid )

    Human FPR1 with F74A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 H57S ( cDNA plasmid )

    Human FPR1 with H57S point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 I204Y ( cDNA plasmid )

    Human FPR1 with I204Y point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 I204Y/R205A ( cDNA plasmid )

    Human FPR1 with I204Y and R205A point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 I224A ( cDNA plasmid )

    Human FPR1 with I224A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 K143A ( cDNA plasmid )

    Human FPR1 with K143A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L101I/F102H ( cDNA plasmid )

    Human FPR1 with L101I and F102H point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L109A ( cDNA plasmid )

    Human FPR1 with L109A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L223A/L240A ( cDNA plasmid )

    Human FPR1 with L223A and L240A point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L243-AAA-S244 ( cDNA plasmid )

    Human FPR1 with L243-AAA-S244 Alanine insertion mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L243A ( cDNA plasmid )

    Human FPR1 with L243A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 L78A ( cDNA plasmid )

    Human FPR1 with L78A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 N297A ( cDNA plasmid )

    Human FPR1 with N297A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 N297A-EGFP ( cDNA plasmid )

    Human FPR1 with N297A point mutation and an EGFP tag. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 N44A ( cDNA plasmid )

    Human FPR1 with N44A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 P213A ( cDNA plasmid )

    Human FPR1 with P213A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 P298A ( cDNA plasmid )

    Human FPR1 with P298A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 Q258A ( cDNA plasmid )

    Human FPR1 with Q258A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R123A ( cDNA plasmid )

    Human FPR1 with R123A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R123G ( cDNA plasmid )

    Human FPR1 with R123G point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R201A ( cDNA plasmid )

    Human FPR1 with R201A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R205A ( cDNA plasmid )

    Human FPR1 with R205A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R241A ( cDNA plasmid )

    Human FPR1 with R241A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 R54A ( cDNA plasmid )

    Human FPR1 with R54A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 S220A ( cDNA plasmid )

    Human FPR1 with S220A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 S287A ( cDNA plasmid )

    Human FPR1 with S287A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 S319L ( cDNA plasmid )

    Human FPR1 with S319L point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 S63C/Y64F ( cDNA plasmid )

    Human FPR1 with S63C/Y64F point mutations. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 S63W ( cDNA plasmid )

    Human FPR1 with S63W point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 T103I ( cDNA plasmid )

    Human FPR1 with T103I point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 T157A ( cDNA plasmid )

    Human FPR1 with T157A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 V105A ( cDNA plasmid )

    Human FPR1 with V105A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 V246A ( cDNA plasmid )

    Human FPR1 with V246A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 W150A ( cDNA plasmid )

    Human FPR1 with W150A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 W254A ( cDNA plasmid )

    Human FPR1 with W254A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 wild-type ( cDNA plasmid )

    Human wild-type FPR1.

  • FPR1 Y221A ( cDNA plasmid )

    Human FPR1 with Y221A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 Y257A ( cDNA plasmid )

    Human FPR1 with Y257A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 Y257F ( cDNA plasmid )

    Human FPR1 with Y257F point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 Y301A ( cDNA plasmid )

    Human FPR1 with Y301A point mutation. Point mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆128-135 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 128-135. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆229-234 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 229-234. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆309-315 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 309-315. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆316-322 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 316-322. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆323-329 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 323-329. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1 ∆330-336 ( cDNA plasmid )

    Human FPR1 with deletion of amino acids 330-336. Deletion mutations of human FPR1 cDNA clone R-26 were generated by oligonucleotide-directed mutagenesis and confirmed by DNA sequencing.

  • FPR1-EGFP ( cDNA plasmid )

    Human FPR1 with EGFP tag. Fusion constructs with EGFP were created as indicated in the linked reference.

  • HA-FPR1 ( cDNA plasmid )

    Human FPR1 with HA tag. Fusion constructs with HA were created as indicated in the linked reference.


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Last updated: 2016-08-25T13:26:19.801-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016