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Merzdorf Lab

Summary:

The lab studies the molecular mechanisms that underlie patterning of the vertebrate nervous system during embryonic development. Our lab focuses on how the early neural plate is subdivided into the different regions of the brain. We use the frog Xenopus and the chick as model organisms.

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Reagents

  • zic1 cDNA clone for antisense RNA probe generation ( Plasmid )

    For zic1, PCR products were synthesized from stage 18 chick cDNA, which was obtained by reverse transcription of isolated total RNA. These PCR products were TA cloned into pGEM-T (Promega) and transcribed from the resulting plasmids to generate antisense RNA probes.

    zic1 forward: 5'-GCGCTAAAACAAAACAGCGA-3'

    zic1 reverse: 5'-CTGTATTTACAAGAGGGAGTGGG-3'
    (497 bp in 3'UTR)

  • zic2 cDNA clone for antisense RNA probe generation ( Plasmid )

    For zic2, PCR products were synthesized from stage 18 chick cDNA, which was obtained by reverse transcription of isolated total RNA. These PCR products were TA cloned into pGEM-T (Promega) and transcribed from the resulting plasmids to generate antisense RNA probes.

    zic2 forward: 5'-CCCTCCTCTCCCTCCTCCT-3'

    zic2 reverse: 5'-ACGCTGATTTCCTCACAACC-3'
    (441 bp in 3'UTR)

  • zic3 antisense RNA probe primers ( Primer )

    T7 promoter-containing PCR products were synthesized from stage 18 chick cDNA. The gel-purified PCR products were used as templates for synthesis of antisense RNA probes using T7 polymerase.

    Note: -TAATACGACTCACTATA- sequence within reverse primer is the T7 RNA polymerase promoter .

  • zic4 antisense RNA probe primers ( Primer )

    T7 promoter-containing PCR products were synthesized from stage 18 chick cDNA. The gel-purified PCR products were used as templates for synthesis of antisense RNA probes using T7 polymerase.

    Note: -TAATACGACTCACTATA- sequence within reverse primer is the T7 RNA polymerase promoter .


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Last updated: 2016-08-25T13:56:53.106-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016