Research in the Harmsen Lab encompasses the areas of Pulmonary Immunology and Immunopathology. The lung is extremely susceptible to infection because of the constant deposition of potential pathogens in the airways that results from breathing contaminated air. Thus, the lung must respond to these pathogens quickly and intensely to avoid infection. Although these host immune and inflammatory responses usually are successful in preventing infection, these processes can also be damaging to the host. This is especially a problem for the lung because the delicate lace-like structure of lung tissue is easily damaged. In addition, misguided immune responses to inhaled noninfectious antigens, such as allergens, can directly cause serious diseases such as asthma and bronchitis. The major goal of our research is to better understand how the lung immune responses can resist infections and yet limit "collateral" host damage caused by the immune response. In the process of studying these mechanisms of resistance and tissue damage, our lab utilizes animal models of disease. These include mouse models of influenza, Pneumocystis murina pneumonia, Coxiella burnetii, and Streptococcus as well as bovine models of viral and bacterial pneumonia.
Unrestrained whole body plethysmography apparatus for evaluating various pulmonary function parameters. In-line nebulizer for aerosol delivery.
For standard sectioning of paraffin-embedded tissues.
Add-on to Nikon Eclipse E800 fluorescent microscope.
Microscope with 2x, 4x ,10x, 20x, 40x, 60x and 100x objectives. FITC, Texas Red, DAPI and FITC/Texas Red dual filters.
"Commonly referred to as BALB/c scid, these animals carry the severe combined immune deficiency mutation (scid) on the BALB/c background."
An opportunisitic pathogen of the lower respiratory tract.
Human alveolar adenocarcinoma cell line.
"The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. Binding of GK1.5 is blocked by RM4-5."
The Harmsen Lab uses this antibody to analyze and deplete CD8 T cell populations in mouse models of infection and immunity.
This antibody targets the mouse CD8 T cell receptor and is used in the Harmsen Lab to analyze and deplete these populations in mouse models of infection and immunity.
These cells are used in the Harmsen Lab to perform influenza viral plaque assays.